A fast impedance-based antimicrobial susceptibility test

Daniel C. Spencer, Teagan F. Paton, Kieran T. Mulroney, Timothy J.J. Inglis, John Sutton, Hywel Morgan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

There is an urgent need to develop simple and fast antimicrobial susceptibility tests (ASTs) that allow informed prescribing of antibiotics. Here, we describe a label-free AST that can deliver results within an hour, using an actively dividing culture as starting material. The bacteria are incubated in the presence of an antibiotic for 30 min, and then approximately 105 cells are analysed one-by-one with microfluidic impedance cytometry for 2–3 min. The measured electrical characteristics reflect the phenotypic response of the bacteria to the mode of action of a particular antibiotic, in a 30-minute incubation window. The results are consistent with those obtained by classical broth microdilution assays for a range of antibiotics and bacterial species.

Original languageEnglish
Article number5328
JournalNature Communications
Volume11
Issue number1
DOIs
Publication statusPublished - 1 Dec 2020

Bibliographical note

Funding Information:
We would like to thank Ying Tran for fabricating the micro-cytometer chips. H.M. would like to thank the Royal Society for funding. T.J.J.I. and D.C.S. acknowledge travel funding from the University of Western Australia.

Publisher Copyright:
© 2020, The Author(s).

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