A faster and easier biodosimetry method based on calyculin A-induced premature chromosome condensation (PCC) by scoring excess objects

Mingzhu Sun*, Jayne Moquet, David Lloyd, Elizabeth Ainsbury

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Dicentric analysis and the ring PCC assay as established biodosimetry methods both have limitations in the estimation of absorbed doses in suspected overexposure cases between 5 and 10 Gy. The proposed method based on calyculin A-induced PCC overcomes these limitations by scoring excess objects as the endpoint. This new scoring method can potentially serve as a faster and up-scalable approach that complements the existing methods with higher accuracy at different dose ranges. It can also potentially be performed by less skilled workers when no automated system is available in mass casualty emergency cases to assist with the triage of patients. Additionally, it offers the possibility to further reduce the sample size and PCC induction time. In this pilot study, a calibration curve for excess objects was constructed using the new scoring method for the first time and a blind validation test composed of three unknown doses was carried out. Almost all the dose estimates were within the 95% confidence limits of the actual test doses by scoring only 50-100 PCC spreads. This method was found to be more accurate than ring PCC for doses below 10 Gy.

Original languageEnglish
Pages (from-to)892-905
Number of pages14
JournalJournal of Radiological Protection
Volume40
Issue number3
DOIs
Publication statusPublished - Sep 2020

Keywords

  • biodosimetry
  • calyculin A-induced PCC
  • dose-response curve
  • excess objects
  • premature chromosome condensation (PCC)

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