A novel real-time polymerase chain reaction for identification of Salmonella enterica subspecies enterica

Katie L. Hopkins, Andrew Lawson, Steve Connell, Tansy Peters, Elizabeth de Pinna

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Salmonella enterica subspecies enterica (subspecies I) causes the majority of infections in humans and homeothermic animals. We present a real-time polymerase chain reaction assay targeting the hilA gene that demonstrates 97.9% specificity and 99.9% sensitivity for rapid and reliable identification of subspecies I, offering savings in time and labor over traditional methods.

Original languageEnglish
Pages (from-to)278-280
Number of pages3
JournalDiagnostic Microbiology and Infectious Disease
Volume70
Issue number2
DOIs
Publication statusPublished - Jun 2011

Keywords

  • 5′ Nuclease real-time PCR
  • HilA
  • Salmonella enterica subspecies enterica

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