A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis)

María Victoria Ortega-García, Francisco Javier Salguero Bodes, Antonio Rodríguez-Bertos, Inmaculada Moreno, Nerea García, Teresa García-Seco, Gabriela Luz Torre, Lucas Domínguez, Mercedes Domínguez

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

In this study, we describe the pathology of Leishmania infantum infection in naturally infected wild Leporidae and compare diagnosis of infection using histopathology, direct fluorescent antibody (DFA) assay, immunofluorescence antibody test (IFAT) and quantitative real-time PCR (qPCR). Tissues were analysed from 52 European rabbits (Oryctolagus cuniculus) and 7 Iberian hares (Lepus granatensis) from the Community of Madrid (Spain). Our results show that L. infantum infection is associated with only minimal histopathological lesions and that L. infantum amastigotes can be detected by DFA assay in all tissues types tested, including skin. These results were confirmed by qPCR on fresh frozen tissues in 13% of rabbits and 100% of hares. However, L. infantum DNA could not be detected by qPCR on paraffin-embedded tissue obtained by laser capture microdissection. Using the DFA assay to diagnose L. infantum, infection may provide further insights into this disease in wild animals and may allow the precise tissue localization of L. infantum, thereby guiding follow-up tests with more accurate qPCR.

Original languageEnglish
Pages (from-to)2474-2481
Number of pages8
JournalTransboundary and Emerging Diseases
Volume66
Issue number6
DOIs
Publication statusPublished - 1 Nov 2019

Keywords

  • diagnostic
  • histopathology
  • immunofluorescence
  • Leishmania infantum
  • wild animals

Fingerprint

Dive into the research topics of 'A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis)'. Together they form a unique fingerprint.

Cite this