A reverse genetics system of African horse sickness virus reveals existence of primary replication

Eiko Matsuo, Cristina Celma, Polly Roy*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

African horse sickness virus (AHSV), a member of the orbivirus genus of the family Reoviridae, is an insect-vectored pathogen of horses of concern to the equine industry. Studies on AHSV replication and pathogenesis have been hampered by the lack of reverse genetics allowing targeted mutation of viral genomes. We demonstrate that AHSV single-stranded RNA synthesized in vitro (core transcripts) is infectious and that there are distinct primary and secondary stages of the replication cycle. Transfection with a mixture of core transcripts from two different serotypes or a mixture of core transcripts and a T7 derived transcript resulted in the recovery of reassortant viruses. Recovery of infectious ASHV from nucleic acid will benefit investigation of the virus and the generation of attenuated vaccines.

Original languageEnglish
Pages (from-to)3386-3391
Number of pages6
JournalFEBS Letters
Volume584
Issue number15
DOIs
Publication statusPublished - Aug 2010
Externally publishedYes

Bibliographical note

Funding Information:
We thank Maria McCrossan (LSHTM) for technical help with electron microscopy. This work was funded by BBSRC (UK) .

Keywords

  • AHSV
  • Core transcript
  • Primary replication
  • Reverse genetic
  • T7 transcript

Fingerprint

Dive into the research topics of 'A reverse genetics system of African horse sickness virus reveals existence of primary replication'. Together they form a unique fingerprint.

Cite this