An assay for botulinum toxin types A, B and F that requires both functional binding and catalytic activities within the neurotoxin

E. R. Evans, P. J.A. Skipper, Clifford Shone

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28 Citations (Scopus)


Aim: To develop a novel assay technique for the botulinum neurotoxin family (BoNTs) which is dependent on both the endopeptidase and receptor-binding activities of the BoNTs and which is insensitive to antigenic variation with the toxin family. Methods and Results: An endopeptidase activity, receptor-binding assay (EARB assay) has been developed which captures biologically active toxin from media using brain synaptosomes. After capture, the bound toxin can be incubated with its substrate, and cleavage detected using serotype-specific antibodies raised against the cleaved product of each toxin serotype. The EARB assay was assessed using a range of BoNT serotypes and subtypes. For BoNT/A, detection limits for subtypes A1, A2 and A3 were 0·5, 3 and 10 MLD50 ml-1, respectively. The limit of detection for BoNT/B1 was 5 MLD50 ml-1 and a novel antibody-based endopeptidase assay for BoNT/F detected toxin at 0·5 MLD50 ml-1. All these BoNTs can be captured from media containing up to 10% serum without loss of sensitivity. BoNT/A 1 could also be detected in dilutions of a lactose- containing formulation similar to that used for clinical preparations of the toxin. Different serotypes were found to possess different optimal cleavage pHs (pH 6·5 for A1, pH 7·4 for B1). Conclusions: The EARB assay has been shown to be able to detect a broad range of BoNT serotypes and subtypes from various media. Significance and Impact of the Study: The EARB assay system described is the first convenient in vitro assay system described which is requires multiple functional biological activities with the BoNTs. The assay will have applications in instances where it is essential or desirable to distinguish biologically active from inactive neurotoxin.

Original languageEnglish
Pages (from-to)1384-1391
Number of pages8
JournalJournal of Applied Microbiology
Issue number4
Publication statusPublished - Oct 2009


  • Activity
  • Detection
  • Identification
  • Mechanism of action
  • Rapid techniques
  • Toxins


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