An assessment of different DNA delivery systems for protection against respiratory syncytial virus infection in the murine model: Gene-gun delivery induces IgG in the lung

Julia Tree, Gary Bembridge, Sam Hou, Geraldine Taylor, Elizabeth Fashola-Stone, José Melero, Martin P. Cranage*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)

    Abstract

    Immunization with plasmid DNA (pDNA) has the potential to overcome the difficulties of neonatal vaccination that may be required for protection against infection with respiratory syncytial virus (RSV); however, little is known about optimal delivery modalities. In this pilot study we compared mucosal delivery of pDNA encoding RSV F protein encapsulated in poly(DL-lactide-co- glycolide) with delivery of pDNA by gene-gun for the induction of immunity in mice. Intra-gastric or intra-nasal immunization with various doses of microparticles induced weak low levels of RSV-specific serum antibodies in a proportion of mice; in contrast, gene-gun vaccination led to protective immunity associated with a humoral response. Interestingly, RSV-specific antibody was detected in lung fragment cultures following intradermal vaccination with the gene-gun.

    Original languageEnglish
    Pages (from-to)2438-2443
    Number of pages6
    JournalVaccine
    Volume22
    Issue number19
    DOIs
    Publication statusPublished - 23 Jun 2004

    Bibliographical note

    Funding Information:
    We are grateful to Lisa Hyland for advice regarding fragment cultures. We thank Peter Beverley for useful discussions. The work was supported by the UK Department of Health. The views expressed in the publication are those of the authors and not necessarily those of the Department of Health.

    Keywords

    • DNA vaccine
    • Lung antibodies
    • Respiratory syncytial virus

    Fingerprint

    Dive into the research topics of 'An assessment of different DNA delivery systems for protection against respiratory syncytial virus infection in the murine model: Gene-gun delivery induces IgG in the lung'. Together they form a unique fingerprint.

    Cite this