An improved protocol for the preparation of total genomic DNA from isolates of yeast and mould using Whatman FTA filter papers

Andrew M. Borman, Mark Fraser, Christopher J. Linton, Michael D. Palmer, Elizabeth Johnson

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Here, we present a significantly improved version of our previously published method for the extraction of fungal genomic DNA from pure cultures using Whatman FTA® filter paper matrix technology. This modified protocol is extremely rapid, significantly more cost effective than our original method, and importantly, substantially reduces the problem of potential cross-contamination between sequential filters when employing FTA technology.

Original languageEnglish
Pages (from-to)445-449
Number of pages5
JournalMycopathologia
Volume169
Issue number6
DOIs
Publication statusPublished - Jun 2010

Keywords

  • DNA extraction
  • FTA technology
  • Fungal genomic DNA
  • Molecular identification
  • PCR

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