Photon correlation spectroscopy demonstrated for the first time that co-purified meningococcal TbpA+B form a complex in solution. This structure bound hTf and the resultant species underwent partial dissociation after exposure to additional hTf or following prolonged incubation. Purified TbpA and TbpB bad similar apparent sizes but showed distinctive size profiles suggesting that TbpA forms a largely homogeneous population while TbpB may produce more variable particle sizes under these conditions.
Bibliographical noteFunding Information:
Acknowledgements." This work was supported by Biotechnology and Biological Sciences Research Council studentship no. 9456368X, CAMR and the Special Trustees for St. Thomas Hospital.
- Neisseria meningitidis
- Photon correlation spectroscopy