Chromosomal aberrations in human lymphocytes induced in vitro by very low doses of x-rays

D. C. Lloyd, Alan Edwards, A. Leonard, G. L. Deknudt, L. Verschaeve, A. T. Natarajan, F. Darroudi, G. Obe, F. Palitti, C. Tanzarella, E. J. Tawn

Research output: Contribution to journalArticlepeer-review

129 Citations (Scopus)

Abstract

This paper presents results of a collaborative experiment between six laboratories which examined the yields of unstable chromosomal aberrations in human lymphocytes induced in vitro by X-rays over the dose range 0-300 mGy. The work included data points of nominal doses of 0, 3, 5, 6, 10, 20, 30, 50 and 300 mGy. Cells from 24 donors were examined and a total of about 300 000 metaphases were scored. The work was undertaken to determine the limits of sensitivity of the system taking into account variations in scoring data due to interdonor sample and inter-laboratory effects. Despite the existence of these effects, aberration yields significantly in excess of control values were seen at doses > 20 mGy and these were consistent with a linear extrapolation from higher doses. Below 20 mGy the observed dicentric yields were generally lower than background, but not significantly so. Excess acentric aberrations, on the other hand, and centric rings, were higher than the controls but the increase was usually not significant. It is concluded that the statistical uncertainties are such that below 20 mGy this technique cannot distinguish between a linear or a threshold model.

Original languageEnglish
Pages (from-to)335-343
Number of pages9
JournalInternational Journal of Radiation Biology
Volume61
Issue number3
DOIs
Publication statusPublished - 1992

Fingerprint

Dive into the research topics of 'Chromosomal aberrations in human lymphocytes induced in vitro by very low doses of x-rays'. Together they form a unique fingerprint.

Cite this