Common oligosaccharide moieties inhibit the adherence of typical and atypical respiratory pathogens

Richard Thomas, Timothy Brooks

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)

Abstract

Intervention in bacterial adhesion to host cells is a novel method of overcoming current problems associated with antibiotic resistance. Antibiotic-resistant strains of bacteria that cause respiratory tract infections are a problem in hospitals and could be used in bioterrorist attacks. A range of bacterial species was demonstrated to attach to an alveolar epithelial (A549) cell line. In all cases, cell surface oligosaccharides were important in attachment, demonstrated by reduced adhesion when A549 cells were pre-treated with tunicamycin. Bacillus anthracis and Yersinia pestis displayed a restricted tropism for oligosaccharides compared to the environmental, opportunistic pathogens, Pseudomonas aeruginosa, Burkholderia cenocepacia. Burkholderia pseudomallei and Legionella pneumophila. The compound with the greatest anti-adhesion activity was p-nitrophenol. Other generic attachment inhibitors included the polymeric saccharides (dextran and heparin), GalNAcβ1-4Gal, GalNAcβ1-3Gal, Galβ1-4GlcNAc and Galβ1-3GlcNAc. Burkholderia pseudomallei attachment was particularly susceptible to oligosaccharide inhibition. Combinations of such compounds may serve as a novel generic therapeutics for respiratory tract infections.

Original languageEnglish
Pages (from-to)833-840
Number of pages8
JournalJournal of Medical Microbiology
Volume53
Issue number9
DOIs
Publication statusPublished - Sep 2004

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