Comparable dose estimates of blinded whole blood samples are obtained independently of culture conditions and analytical approaches. Second RENEB gene expression study

Grainne Manning, Ellina Macaeva, Matthaeus Majewski, Ralf Kriehuber, Kamil Brzóska, Michael Abend, Sven Doucha-Senf, Dominik Oskamp, Sonja Strunz, Roel Quintens, Matthias Port, Christophe Badie*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Purpose: This collaboration of five established European gene expression labs investigated the potential impact of culture conditions on the transcriptional response of peripheral blood to radiation exposure. Materials and methods: Blood from one healthy donor was exposed ex vivo to a Cobalt 60 source to produce a calibration curve in addition to four unknown doses. After exposure, the blood samples were either diluted with RPMI medium or left untouched. After 24-h incubation at 37 °C the diluted blood samples were lysed, while the undiluted samples were mixed with the preservative RNALater and all samples were shipped frozen to the participating labs. Samples were processed by each lab using microarray (one lab) and QRT-PCR (four labs). Results: We show that although culture conditions affect the total amount of RNA recovered (p <.0001) and its integrity (p <.0001), it does not significantly affect dose estimates (except for the true dose at 1.1 Gy). Most importantly, the different analysis approaches provide comparable mean absolute difference of estimated doses relative to the true doses (p =.9) and number of out of range (>0.5 Gy) measurements (p =.6). Conclusion: This study confirms the robustness of gene expression as a method for biological dosimetry.

Original languageEnglish
Pages (from-to)87-98
Number of pages12
JournalInternational Journal of Radiation Biology
Volume93
Issue number1
DOIs
Publication statusPublished - 2 Jan 2017

Bibliographical note

Publisher Copyright:
© 2016 Crown Copyright. Published by Informa UK Limited, trading as Taylor & Francis Group.

Keywords

  • Biological dosimetry
  • biomarkers
  • dose-response
  • gene expression
  • ionising radiation
  • peripheral blood

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