Candida auris is an emerging worldwide concern, but comparative data about the virulence of different C. auris lineages in mammalian hosts is lacking. Different isolates of the four prevalent C. auris clades (South Asian n = 5, East Asian n = 4, South African n = 5, and South American n = 5) were compared to assess their virulence in a neutropenic murine bloodstream infection model with C. albicans as reference. C. auris, regardless of clade, proved to be less virulent than C. albicans. Highest overall mortality at day 21 was observed for the South American clade (96%), followed by the South Asian (80%), South African (45%) and East Asian (44%) clades. Fungal burden results showed close correlation with lethality. Histopathological examination revealed large aggregates of blastoconidia and budding yeast cells in the hearts, kidneys and livers but not in the spleens. The myocardium of apparently healthy sacrificed mice as well as of mice found moribund showed contraction band necrosis in case of all lineages. Regardless of clade, the heart and kidneys were the most heavily affected organs. Isolates of the same clade showed differences in virulence in mice, but a markedly higher virulence of the South American clade was clearly demonstrated.
Bibliographical noteFunding Information: Z. T. and F. N. were supported by the EFOP-3.6.3-VEKOP-16–2017-00009 Program. R. K. was supported by the TA ´MOP 4.2.4.A/2–11-1–2012-0001 National Excellence Program (Elaborating and operating an inland student and researcher personal support system). The project was subsidized by the European Union and co-ﬁnanced by the European Social Fund. Z. T. and F. N. were supported by the U ´NKP-19–3 New National Excellence Program of the Ministry of Human Capacities.
L. Majoros received conference travel grants fromMSD, Astellas, Pfizer and Cidara.
Open Access: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Publisher Copyright: © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd.
Citation: Lajos Forgács, Andrew M. Borman, Eszter Prépost, Zoltán Tóth, Gábor Kardos, Renátó Kovács, Adrien Szekely, Fruzsina Nagy, Ilona Kovacs & László Majoros (2020) Comparison of in vivo pathogenicity of four Candida auris clades in a neutropenic bloodstream infection murine model, Emerging Microbes & Infections, 9:1, 1160-1169.
- aggregating Candida auris
- contraction band necrosis
- fungal tissue burden
- myocardial involvement