Comparison of large-scale mammalian cell culture systems with egg culture for the production of influenza virus A vaccine strains

Julia Tree*, Catherine Richardson, Anthony R. Fooks, J. Christopher Clegg, Denis Looby

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    126 Citations (Scopus)

    Abstract

    Different types of microcarriers were assessed for the large-scale culture of influenza virus in the Madin-Darby canine kidney (MDCK) cells. Both porous and solid carriers were examined. A higher titre of influenza A/PR8/34 virus was recovered from cultures using solid (1.3 × 109 PFU per ml) rather than porous carriers (4.0 × 108 PFU per ml). High titres of virus (1.0 × 109 PFU per ml) were also obtained from roller bottle cultures of MDCK cells and the traditional culture technique using embryonated hens' eggs (3.9 × 109 PFU per ml). We found that solid carriers composed of dextran with a positive charge are the most suitable carriers for the large-scale growth of influenza A virus in MDCK cells using serum-free media. Crown

    Original languageEnglish
    Pages (from-to)3444-3450
    Number of pages7
    JournalVaccine
    Volume19
    Issue number25-26
    DOIs
    Publication statusPublished - 14 May 2001

    Bibliographical note

    Funding Information:
    This work was funded by the Department of Health, UK. Egg-adapted human influenza reassortant, prototype A/PR8/34 was obtained from Dr J Wood, NIBSC for which we are very grateful. We are grateful for the technical assistance of Mrs Hilary House. We would also like to thank Dr Ian Brown (VLA, Weybridge, Surrey) and Dr Maria Zambon (PHLS, Colindale, London) for critically reading this manuscript.

    Keywords

    • Influenza
    • MDCK
    • Mammalian cell-culture
    • Microcarrier
    • Vaccine

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