Different types of microcarriers were assessed for the large-scale culture of influenza virus in the Madin-Darby canine kidney (MDCK) cells. Both porous and solid carriers were examined. A higher titre of influenza A/PR8/34 virus was recovered from cultures using solid (1.3 × 109 PFU per ml) rather than porous carriers (4.0 × 108 PFU per ml). High titres of virus (1.0 × 109 PFU per ml) were also obtained from roller bottle cultures of MDCK cells and the traditional culture technique using embryonated hens' eggs (3.9 × 109 PFU per ml). We found that solid carriers composed of dextran with a positive charge are the most suitable carriers for the large-scale growth of influenza A virus in MDCK cells using serum-free media. Crown
Bibliographical noteFunding Information:
This work was funded by the Department of Health, UK. Egg-adapted human influenza reassortant, prototype A/PR8/34 was obtained from Dr J Wood, NIBSC for which we are very grateful. We are grateful for the technical assistance of Mrs Hilary House. We would also like to thank Dr Ian Brown (VLA, Weybridge, Surrey) and Dr Maria Zambon (PHLS, Colindale, London) for critically reading this manuscript.
- Mammalian cell-culture