Development and evaluation of a rapid multianalyte particle-based flow cytometric assay for the quantification of meningococcal serogroup B-specific IgM antibodies in sera for nonculture case confirmation

Gouri Laher, Paul Balmer, Steve J. Gray, Maureen Dawson, Edward Kaczmarski, Raymond Borrow

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Serogroup-specific antibody has been shown to be present in the sera of patients recovering from meningococcal disease, and thus the detection of such antibodies may aid in the confirmation of disease. There are currently no standard methods for measuring meningococcal serogroup B-specific antibody in sera. Here, we report the development of a microsphere-based immunoassay which utilizes colominic acid from Escherichia coli 07:K1 (L):NM to detect immunoglobulin M directed against serogroup B polysaccharide. The serogroup B assay was incorporated into a multiplex assay which also detects serogroup-specific immunoglobulin M for meningococcal serogroups A, C, Y and W-135. Using the method of cross-standardization, serogroup B-specific immunoglobulin M concentrations were assigned to the standard serum CDC 1992. The assay is able to detect increases in specific immunoglobulin M concentrations from acute to convalescent phase serum from serogroup B cases, and can be utilized in conjunction with the previously developed tetraplex immunoglobulin G detection assay for serogroups A, C, Y and W-135.

Original languageEnglish
Pages (from-to)34-43
Number of pages10
JournalFEMS Immunology and Medical Microbiology
Volume48
Issue number1
DOIs
Publication statusPublished - Oct 2006

Keywords

  • Flow cytometric assay
  • Meningococcal
  • Serogroup B

Fingerprint

Dive into the research topics of 'Development and evaluation of a rapid multianalyte particle-based flow cytometric assay for the quantification of meningococcal serogroup B-specific IgM antibodies in sera for nonculture case confirmation'. Together they form a unique fingerprint.

Cite this