Development of a guinea pig immune response-related microarray and its use to define the host response following Mycobacterium bovis BCG vaccination

Julia Tree, Michael Elmore, Sajid Javed, Ann Williams, Phillip Marsh

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Immune responses in the guinea pig model are understudied because of a lack of commercial reagents. We have developed a custom-made guinea pig oligonucleotide microarray (81 spots) and have examined the gene expression profile of splenocytes restimulated in vitro from Mycobacterium bovis BCG-vaccinated and naive animals. Eleven genes were significantly (P < 0.05) up-regulated following vaccination, indicating a Th1-type response. These results show that microarrays can be used to more fully define immune profiles of guinea pigs.

Original languageEnglish
Pages (from-to)1436-1441
Number of pages6
JournalInfection and Immunity
Volume74
Issue number2
DOIs
Publication statusPublished - Feb 2006

Fingerprint

Dive into the research topics of 'Development of a guinea pig immune response-related microarray and its use to define the host response following Mycobacterium bovis BCG vaccination'. Together they form a unique fingerprint.

Cite this