Development of a real-time RT-PCR assay for the detection of Crimean-Congo hemorrhagic fever virus

Barry Atkinson, John Chamberlain, Christopher H. Logue, Nicola Cook, Christine Bruce, Stuart Dowall, Roger Hewson

Research output: Contribution to journalArticlepeer-review

66 Citations (Scopus)

Abstract

Crimean-Congo hemorrhagic fever (CCHF) is a virulent tick-borne disease with a case fatality rate ranging from 10-50% for tick-borne transmission, and up to 80% for nosocomial transmission. Human cases have been reported in over 30 countries across Europe, Asia, and Africa. It appears to be spreading to new areas with several countries reporting their first human cases of CCHF disease within the past 10 years. We report a novel real-time RT-PCR assay designed to amplify a conserved region of the CCHF virus S segment. It is capable of detecting strains from all 7 groups of CCHF, including the AP92 strain that until recently represented a lineage of strains that were not associated with human disease. The limit of detection of the assay is 5 copies of target RNA, and the assay shows no cross-reactivity with other viruses from within the same genus, or with viruses causing similar human disease.

Original languageEnglish
Pages (from-to)786-793
Number of pages8
JournalVector-Borne and Zoonotic Diseases
Volume12
Issue number9
DOIs
Publication statusPublished - 1 Sep 2012

Keywords

  • Assay
  • CCHF
  • Crimean-Congo Hemorrhagic Fever
  • Detection
  • Real-time RT-PCR

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