Differentiation of Mycobacterium tuberculosis complex and nontuberculous mycobacterial liquid cultures by using peptide nucleic acid-fluorescence in situ hybridization probes

Francis Drobniewski*, P. G. More, G. S. Harris

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

A blinded comparison of peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) with routine identification methods was performed on 74 consecutively positive mycobacterial liquid cultures. All Mycobacterium tuberculosis cultures (48 of 48) and 22 of 27 (81.5%) nontuberculous cultures were correctly identified (including one mixed culture). Five isolates yielded no reaction with either probe and were identified as Mycobacterium xenopi, Mycobacterium fortuitum, or Mycobacterium flavescens.

Original languageEnglish
Pages (from-to)444-447
Number of pages4
JournalJournal of Clinical Microbiology
Volume38
Issue number1
Publication statusPublished - 2000

Bibliographical note

Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.

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