Early (2008-2010) hospital outbreak of Klebsiella pneumoniae producing OXA-48 carbapenemase in the UK

Claire P. Thomas, Luke S.P. Moore*, Nazik Elamin, Michel Doumith, Jiancheng Zhang, Sunil Maharjan, Marina Warner, Claire Perry, Jane Turton, Clare Johnstone, Annette Jepson, Neill D.C. Duncan, Alison H. Holmes, David M. Livermore, Neil Woodford

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

OXA-48 β-lactamase is one of the several emerging carbapenemases. Pre-2007 reports were almost exclusively from Turkey, but subsequently its distribution has expanded. We report an early and prolonged outbreak in the UK of Klebsiella pneumoniae producing OXA-48 carbapenemase affecting a predominantly renal cohort in a West London hospital. Carbapenemase production was detected by the modified Hodge test, with confirmation by PCR for bla OXA-48. Isolates were typed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Risk factors for acquisition were determined. Between January 2008 and April 2010, 20 K. pneumoniae isolates with reduced susceptibility to carbapenems were identified from 13 patients, comprising 12 renal cases and 1 oncology patient; 8 were outpatients and 5 were inpatients; 7 were deemed to be colonised and 6 infected, including 2 with bacteraemia, 1 of whom died. Hodge tests were positive for all isolates and all had blaOXA-48. PFGE showed strain similarity in isolates from nine patients, whereas four patients' isolates were distinct, representing three further PFGE profiles and suggesting horizontal spread of blaOXA-48. Most patients had received antibiotics in the preceding 3 months and all had healthcare contact, but none had recent travel to areas with endemic OXA-48 Enterobacteriaceae. The renal cohort was screened and a prevalence rate of 0.17% was found. Interventions that collectively brought the outbreak under control included strict infection control precautions, screening, improved laboratory detection protocols and antibiotic stewardship rounds.

Original languageEnglish
Pages (from-to)531-536
Number of pages6
JournalInternational Journal of Antimicrobial Agents
Volume42
Issue number6
DOIs
Publication statusPublished - Dec 2013

Bibliographical note

Funding Information:
The authors thank Prof. Patrice Nordmann for representatives of Turkish clones A and B. Funding : NE was supported for her MSc by a grant from the Special Trustees of Imperial College Healthcare NHS Trust . LSPM is funded by a National Institute for Health Research Imperial Biomedical Research Centre Clinical Research Training Fellowship , and both LSPM and AHH are affiliated with the Centre for Infection Prevention & Management, which is funded by the UK Clinical Research Collaboration . Competing interests : CPT has received travel sponsorship from Novartis. DML is partly self-employed and consults for numerous pharmaceutical and diagnostic companies, including Achaogen, Adenium, Allecra, Astellas, AstraZeneca, Bayer, Basilea, bioMérieux, Cubist, Curetis, Discuva, Fedora, GSK, Merck, Meiji Seika, Pfizer, Roche, Shionogi, Tetraphase, VenatoRx and Wockhardt; he holds grants or contracts from AstraZeneca, Cubist, Meiji Seika, Merck and Wockhardt; has received lecture honoraria or travel reimbursement from AstraZeneca, Bruker, Curetis, GSK, J&J, Merck, Novartis, Pfizer and Tetraphase; and holds shares in Dechra, Eco Animal Health, GSK, Merck and Pfizer, collectively amounting to <10% of portfolio value. AHH has consulted for bioMérieux. All other authors declare no competing interests. Ethical approval : Not required.

Keywords

  • Class D β-lactamase
  • Infection control
  • Renal
  • Sequence type

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