Epidemiological characterization of methicillin-resistant Staphylococcus aureas isolated in the North West of England by protein A (spa) and coagulase (coa) gene polymorphisms

J. Walker, Raymond Borrow*, V. Edwards-Jones, B. A. Oppenheim, Andrew Fox

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

In a comparative study, isolates of methicillin-resistant Staphylococcus aureas (MRSA) with known pulsed-field gel electrophoresis (PFGE) and bacteriophage type were analysed by polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP) for additional discriminatory subtyping information. PFGE was previously performed using standardized, commercially available kits and pre-programmed software. Isolates were examined for coagulase (coa) and protein A (spa) gene polymorphisms following PCR amplification of the coa hypervariable and spa repeat regions. Coa gene RFLPs produced a total of 38 distinct combined patterns after digestion with HaeIII and AluI and identified the predominant epidemic (EMRSA) types 15 and 16. A unique HaeIII restriction site was identified by RFLP and sequence analysis in the coa gene for EMRSA 15 but not EMRSA 16. The spa gene PCR yielded a total of 14 different profiles ranging from 3-18 repeats with the 2 predominant EMRSA types falling into 2 distinct groups. PCR detection of coa and spa polymorphisms offer a rapid preliminary strain identification and discriminatory subtyping information for surveillance of MRSA.

Original languageEnglish
Pages (from-to)507-514
Number of pages8
JournalEpidemiology and Infection
Volume121
Issue number3
DOIs
Publication statusPublished - 1998
Externally publishedYes

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