Escherichia coli sequence type 410 is causing new international high-risk clones

Louise Roer, Søren Overballe-Petersen, Frank Hansen, Kristian Schønning, Mikala Wang, Bent L. Røder, Dennis S. Hansen, Ulrik S. Justesen, Leif P. Andersen, David Fulgsang-Damgaard, Katie L. Hopkins, Neil Woodford, Linda Falgenhauer, Trinad Chakraborty, Ørjan Samuelsen, Karin Sjöström, Thor B. Johannesen, Kim Ng, Jens Nielsen, Steen EthelbergMarc Stegger, Anette M. Hammerum*, Henrik Hasman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

66 Citations (Scopus)

Abstract

Escherichia coli sequence type 410 (ST410) has been reported worldwide as an extraintestinal pathogen associated with resistance to fluoroquinolones, third-generation cephalosporins, and carbapenems. In the present study, we investigated national epidemiology of ST410 E. coli isolates from Danish patients. Furthermore, E. coli ST410 was investigated in a global context to provide further insight into the acquisition of the carbapenemase genes blaOXA-181 and blaNDM-5 of this successful lineage. From 127 whole-genome-sequenced isolates, we reconstructed an evolutionary framework of E. coli ST410 which portrays the antimicrobial-resistant clades B2/H24R, B3/H24Rx, and B4/H24RxC. The B2/H24R and B3/H24Rx clades emerged around 1987, concurrently with the C1/H30R and C2/H30Rx clades in E. coli ST131. B3/H24Rx appears to have evolved by the acquisition of the extendedspectrum lactamase (ESBL)-encoding gene blaCTX-M-15 and an IncFII plasmid, encoding IncFIA and IncFIB. Around 2003, the carbapenem-resistant clade B4/H24RxC emerged when ST410 acquired an IncX3 plasmid carrying a blaOXA-181 carbapenemase gene. Around 2014, the clade B4/H24RxC acquired a second carbapenemase gene, blaNDM-5, on a conserved IncFII plasmid. From an epidemiological investigation of 49 E. coli ST410 isolates from Danish patients, we identified five possible regional outbreaks, of which one outbreak involved nine patients with blaOXA-181- and blaNDM-5-carrying B4/H24RxC isolates. The accumulated multidrug resistance in E. coli ST410 over the past two decades, together with its proven potential of transmission between patients, poses a high risk in clinical settings, and thus, E. coli ST410 should be considered a lineage with emerging "high-risk" clones, which should be monitored closely in the future.

Original languageEnglish
Article numbere00337-18
JournalmSphere
Volume3
Issue number4
DOIs
Publication statusPublished - 1 Jul 2018

Bibliographical note

Funding Information:
This work was supported by the Danish Ministry of Health and Prevention as part of the Integrated Surveillance of ESBL/AmpC-producing E. coli and Carbapenemase-Producing Bacteria and by the Scandinavian Society for Antimicrobial Chemotherapy Foundation grant number SLS-588921.

Publisher Copyright:
© 2018 Roer et al.

Keywords

  • BEAST
  • Epidemiology
  • Escherichia coli
  • Evolution
  • High-risk clone
  • Outbreak

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