Estimating partial-body ionizing radiation exposure by automated cytogenetic biodosimetry

Ben C. Shirley, Joan H.M. Knoll, Jayne Moquet, Elizabeth Ainsbury, Ngoc Duy Pham, Farrah Norton, Ruth C. Wilkins, Peter K. Rogan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Purpose: Inhomogeneous exposures to ionizing radiation can be detected and quantified with the dicentric chromosome assay (DCA) of metaphase cells. Complete automation of interpretation of the DCA for whole-body irradiation has significantly improved throughput without compromising accuracy, however, low levels of residual false positive dicentric chromosomes (DCs) have confounded its application for partial-body exposure determination. Materials and methods: We describe a method of estimating and correcting for false positive DCs in digitally processed images of metaphase cells. Nearly all DCs detected in unirradiated calibration samples are introduced by digital image processing. DC frequencies of irradiated calibration samples and those exposed to unknown radiation levels are corrected subtracting this false positive fraction from each. In partial-body exposures, the fraction of cells exposed, and radiation dose can be quantified after applying this modification of the contaminated Poisson method. Results: Dose estimates of three partially irradiated samples diverged 0.2–2.5 Gy from physical doses and irradiated cell fractions deviated by 2.3%–15.8% from the known levels. Synthetic partial-body samples comprised of unirradiated and 3 Gy samples from 4 laboratories were correctly discriminated as inhomogeneous by multiple criteria. Root mean squared errors of these dose estimates ranged from 0.52 to 1.14 Gy2 and from 8.1 to 33.3%2 for the fraction of cells irradiated. Conclusions: Automated DCA can differentiate whole- from partial-body radiation exposures and provides timely quantification of estimated whole-body equivalent dose.

Original languageEnglish
Pages (from-to)1492-1503
Number of pages12
JournalInternational Journal of Radiation Biology
Volume96
Issue number11
DOIs
Publication statusPublished - 1 Nov 2020

Bibliographical note

Funding Information:
CytoGnomix, PHE, and DNRI acknowledge sponsorship by the International Atomic Energy Agency under Coordinated Research Project ?E35010?, entitled ?Applications of Biological Dosimetry Methods in Radiation Oncology, Nuclear Medicine, and Diagnostic and Interventional Radiology (MEDBIODOSE)?. PHE is grateful to H. Thierens, A. Vraal, and V. Vanderickel for irradiating blood samples and MULTIBIODOSE (EU FP7/2007?2013, agreement No. 241536) for support.

Publisher Copyright:
© Copyright © 2020 Taylor & Francis Group LLC.

Keywords

  • Ionizing radiation
  • biodosimetry
  • chromosomal aberrations
  • inhomogeneous exposure
  • software automation

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