Estimation of ELISA results using a parallel curve analysis

Francis Bursa, Ann Yellowlees, Alka Bishop, Angela Beckett, Bassam Hallis, Mary Matheson

Research output: Contribution to journalArticlepeer-review

Abstract

We introduce a new method for the analysis of enzyme-linked immunosorbent assay (ELISA) data. The new method can use data near the asymptotes and does not give undue weight to responses on the flatter parts of the dose-response curve. We apply it to simulated data and to two real-world assays and show it is more accurate and more precise than the traditional interpolation method. In particular, the new method works much better for very low-concentration samples for which the traditional method is often unable to give a result.

Original languageEnglish
Article number112836
JournalJournal of Immunological Methods
Volume486
DOIs
Publication statusPublished - Nov 2020

Keywords

  • Concentration estimation
  • ELISA
  • ELISA statistics
  • Immunoassay

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