European multicenter evaluation of commercial enzyme immunoassays for detecting norovirus antigen in fecal samples

James Gray, Evelyne Kohli, Franco M. Ruggeri, Harry Vennema, Alicia Sánchez-Fauquier, Eckart Schreier, Christopher Gallimore, Miren Iturriza-Go'Mara, Helene Giraudon, Pierre Pothier, Ilaria Di Bartolo, Nadia Inglese, Erwin De Bruin, Bas Van Der Veer, Silvia Moreno, Vanessa Montero, Marí C. De Llano, Marina Höhne, Sabine M. Diedrich

Research output: Contribution to journalArticlepeer-review

80 Citations (Scopus)

Abstract

A total of 2,254 fecal samples were tested in a European multicenter evaluation of commercially available norovirus antigen detection assays. Two commercial enzyme immunoassays, IDEIA Norovirus (Oxoid; Thermo Fisher Scientific, Ely, United Kingdom) and RIDASCREEN Norovirus (R-Biopharm, Darmstadt, Germany), were included in the evaluation, and their performance was compared with the results of reverse transcription-PCR (RT-PCR). Included in the evaluation were samples collected in sporadic cases of gastroenteritis, samples from outbreaks in which two or more samples were collected, well-characterized samples representing genotypes currently cocirculating within Europe, and samples collected from patients with gastroenteritis caused by a pathogen other than norovirus. The sensitivities and specificities of the IDEIA Norovirus and RIDA-SCREEN Norovirus assays were 58.93 and 43.81% and 93.91 and 9637%, respectively, compared with RT-PCR. The sensitivities of both assays for outbreak investigations improved when six or more samples from an outbreak were examined. The IDEIA Norovirus assay exhibited reactivity to a broader range of norovirus genotypes than the RIDASCREEN Norovirus assay, which showed genotype-dependent sensitivities. The results indicate that, if used, these assays should serve as screening assays and the results should be confirmed by RT-PCR.

Original languageEnglish
Pages (from-to)1349-1355
Number of pages7
JournalClinical and Vaccine Immunology
Volume14
Issue number10
DOIs
Publication statusPublished - Oct 2007

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