Evaluating controlled human malaria infection in Kenyan adults with varying degrees of prior exposure to Plasmodium falciparum using sporozoites administered by intramuscular injection

Susanne H. Hodgson*, Elizabeth Juma, Amina Salim, Charles Magiri, Domtila Kimani, Daniel Njenga, Alfred Muia, Andrew O. Cole, Caroline Ogwang, Ken Awuondo, Brett Lowe, Marianne Munene, Peter F. Billingsley, Eric R. James, Anusha Gunasekera, B. Kim L. Sim, Patricia Njuguna, Thomas W. Rampling, Adam Richman, Yonas AbebeGathoni Kamuyu, Michelle Muthui, Sean C. Elias, Sassy Molyneux, Stephen Gerry, Alex Macharia, Thomas N. Williams, Peter C. Bull, Adrian V.S. Hill, Faith H. Osier, Simon J. Draper, Philip Bejon, Stephen L. Hoffman, Bernhards Ogutu, Kevin Marsh

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

68 Citations (Scopus)

Abstract

Background: Controlled human malaria infection (CHMI) studies are a vital tool to accelerate vaccine and drug development. As CHMI trials are performed in a controlled environment, they allow unprecedented, detailed evaluation of parasite growth dynamics (PGD) and immunological responses. However, CHMI studies have not been routinely performed in malaria-endemic countries or used to investigate mechanisms of naturally-acquired immunity (NAI) to Plasmodium falciparum. Methods: We conducted an open-label, randomized CHMI pilot-study using aseptic, cryopreserved P. falciparum sporozoites (PfSPZ Challenge) to evaluate safety, infectivity and PGD in Kenyan adults with low to moderate prior exposure to P. falciparum (Pan African Clinical Trial Registry: PACTR20121100033272). Results: All participants developed blood-stage infection confirmed by quantitative polymerase chain reaction (qPCR). However one volunteer (110) remained asymptomatic and blood-film negative until day 21 post-injection of PfSPZ Challenge. This volunteer had a reduced parasite multiplication rate (PMR) (1.3) in comparison to the other 27 volunteers (median 11.1). A significant correlation was seen between PMR and screening anti-schizont Enzyme Linked Immunosorbent Assays (ELISA) OD (p = 0.044, R = -0.384) but not when volunteer 110 was excluded from the analysis (p = 0.112, R = -0.313). Conclusions: PfSPZ Challenge is safe and infectious in malaria-endemic populations and could be used to assess the efficacy of malaria vaccines and drugs in African populations. Whilst our findings are limited by sample size, our pilot study has demonstrated for the first time that NAI may impact on PMR post-CHMI in a detectable fashion, an important finding that should be evaluated in further CHMI studies.

Original languageEnglish
Article number686
JournalFrontiers in Microbiology
Volume5
Issue numberDEC
DOIs
Publication statusPublished - 2014
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2014 Hodgson, Juma, Salim, Magiri, Kimani, Njenga, Muia, Cole, Ogwang, Awuondo, Lowe, Munene, Billingsley, James, Gunasekera, Sim, Njuguna, Rampling, Richman, Abebe, Kamuyu, Muthui, Elias, Molyneux, Gerry, Macharia, Williams, Bull, Hill, Osier, Draper, Bejon, Hoffman, Ogutu and Marsh.

Keywords

  • Challenge
  • CHMI
  • Falciparum
  • Immunity
  • Malaria

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