Evaluation of the Applied Biosystems automated Taqman polymerase chain reaction system for the detection of meningococcal DNA

Malcolm Guiver, Raymond Borrow, John Marsh, Stephen J. Gray, Edward Kaczmarski, Paul Boseley, David Howells, Andrew J. Fox

Research output: Contribution to journalArticlepeer-review

131 Citations (Scopus)

Abstract

Previous studies on the localization of several different Helicobacter pylori antigens have been contradictory. We have therefore examined by using both one- and two-color flow cytometry (FCM), immunofluorescence (IF), and immunoelectron microscopy (IEM), the possible surface localization of some H. pylori antigens that may be important virulence factors. All four methods detected the lipopolysaccharide and the N-acetyl-neuroaminyllactose-binding hemagglutinin protein (HpaA) as surface-exposed, while the urease enzyme was not detected at all and the neutrophil activating protein only in low concentration on the surface of the H. pylori bacteria during culture of H. pylori in liquid broth for 11 days. The FCM analysis was found to be quite sensitive and specific and also extremely fast compared with IF and IEM, and therefore the preferred method for detection of surface-localized antigens of H. pylori.

Original languageEnglish
Pages (from-to)173-179
Number of pages7
JournalFEMS Immunology and Medical Microbiology
Volume30
Issue number3
DOIs
Publication statusPublished - 1 Jun 2000
Externally publishedYes

Keywords

  • Antigen
  • Flow cytometry
  • Helicobacter pylori
  • Surface localization

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