Evidence for Msh2 haploinsufficiency in mice revealed by MNU-induced sister-chromatid exchange analysis

Simon Bouffler, N. Hofland, Roger Cox, R. Fodde

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The role of Msh2 in chromosome stability has been investigated in a targeted mouse model for HNPCC, Msh2Δ7N. Chromosome aberration frequencies were similar in bone marrow of Msh2(+/+), Msh2(+/-) and Msh2(-/-) mice and no differential effects of in vivo X-irradiation were noted. By contrast, the induction of sister-chromatid exchanges (SCEs) by methyl nitrosourea (MNU) was reduced in Msh2(-/-) and Msh2(+/-) cells to ~20% and ~45% wild-type levels respectively indicating a phenotypic effect of haploinsufficiency of the mouse Msh2 gene. (C) 2000 Cancer Research Campaign.

Original languageEnglish
Pages (from-to)1291-1294
Number of pages4
JournalBritish Journal of Cancer
Volume83
Issue number10
DOIs
Publication statusPublished - 2000

Keywords

  • Alkylating agent
  • Ionizing radiation
  • Mismatch repair
  • Msh2
  • Sister-chromatid exchange

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