First identification of blaOXA-51-like in non-baumannii acinetobacter spp.

Yi Tzu Lee, Jane Turton, Te Li Chen, R. Chen Chih Wu, Wei Che Chang, Chang Phone Fung, Chien Pei Chen, Wen Long Cho, Li Yueh Huang, L. K. Siu

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

BlaOXA-51-like, intrinsic carbapenemase gene in Acinetobacter baumannii previously found only in this species, was detected in a clinical isolate of Acinetobacter genomic species 13TU. This study aimed to characterize this gene in the isolate. Genomic species identification was confirmed by amplified ribosomal DNA restriction analysis and sequence analysis of 16S-23S ribosomal DNA intergenic spacer, rpoB and recA. The blaOXA-51-like gene, with an upstream IS Aba 1 insertion, was plasmid-encoded and the surrounding sequences suggested that its origin was from A. baumannii. Transformation of Acinetobacter genomic species 13TTJ ATCC 17903 with recombinant plasmid bearing ISAbal-blaOXA-51-like from the isolate increased the minimum inhibitory concentrations (MICs) of meropenem and imipenem 256-fold. This is the first report of blaOXA-51-like in an organism other than A. baumannii. This plasmid-borne blaOxA-51-like gene witn an upstream IS Aba 1 insertion confers a high level of carbapenem resistance to Acinetobacter genomic species 13TU.

Original languageEnglish
Pages (from-to)514-520
Number of pages7
JournalJournal of Chemotherapy
Volume21
Issue number5
DOIs
Publication statusPublished - Oct 2009

Keywords

  • Acinetobacter baumannii
  • Non-baumannii Acinetobacter spp.

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