Genomic and Epidemiological Evidence of a Dominant Panton-Valentine Leucocidin-Positive Methicillin Resistant Staphylococcus aureus Lineage in Sri Lanka and Presence among Isolates from the United Kingdom and Australia

Sharla M. McTavish, Sarah J. Snow, Ellie C. Cook, Bruno Pichon, Sarah Coleman, Geoffrey W. Coombs, Stanley Pang, Cesar A. Arias, Lorena Díaz, Emma Boldock, Steve Davies, Mangala Udukala, Angela Kearns, Sisira Siribaddana, Thushan I. De Silva*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Objective: To undertake the first detailed genomic analysis of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Sri Lanka. Methods: A prospective observational study was performed on 94 MRSA isolates collected over a 4 months period from the Anuradhapura Teaching Hospital, Sri Lanka. Screening for mecA, mecC, and the Panton-Valentine leucocidin (PVL)-associated lukS-PV/lukF-PV genes and molecular characterization by spa typing was undertaken. Whole genome sequencing (WGS) and phylogenetic analysis was performed on selected multilocus sequence type (MLST) clonal complex 5 (CC5) isolates from Sri Lanka, England, Australia, and Argentina. Results: All 94 MRSA harbored the mecA gene. Nineteen spa types belonging to nine MLST clonal complexes were identified. Where origin of the sample was recorded, most isolates were from skin and soft tissue infections (70/91; 76.9%), with fewer causing bacteremia (16/91; 17.6%), empyema (3/91; 3.3%) and osteomyelitis (2/91; 2.2%). Sixty two (65.9%) isolates were PVL positive with the majority (56 isolates; 90.3%) belonging to a dominant CC5 lineage. This lineage, PVL-positive ST5-MRSA-IVc, was associated with both community and hospital-onset infections. Based on WGS, representative PVL-positive ST5-MRSA-IVc isolates from Sri Lanka, England and Australia formed a single phylogenetic clade, suggesting wide geographical circulation. Conclusions: We present the most detailed genomic analysis of MRSA isolated in Sri Lanka to date. The analysis identified a PVL-positive ST5-MRSA-IVc that is prevalent among MRSA causing clinical infections in Sri Lanka. Furthermore, this clone was also found among isolates from the United Kingdom and Australia.

Original languageEnglish
Article number123
JournalFrontiers in Cellular and Infection Microbiology
Volume9
Issue numberAPR
DOIs
Publication statusPublished - 2019

Bibliographical note

Funding Information:
TdS is funded by a Wellcome Trust Intermediate Clinical Fellowship (110058/Z/15/Z). LD was supported by grants COL130871250417 and COL130874455850 from Colciencias. CA is funded through an NIH-NIAID grant K24 AI-AI121296, R01-AI134637, R01-AI093749 and R21 AI121519. During fieldwork, SJS and EC were supported by student grants from The Sheffield Medico-Chirurgical Society, The Sheffield Grammer School’ Trust and The 800th Lord Mayor’s Anniversary Trust.

Publisher Copyright:
Copyright © 2019 McTavish, Snow, Cook, Pichon, Coleman, Coombs, Pang, Arias, Díaz, Boldock, Davies, Udukala, Kearns, Siribaddana and de Silva. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Keywords

  • CC5
  • Methicillin resistant Staphylococcus aureus
  • Panton-Valentine leucocidin
  • Sri Lanka
  • Whole genome sequencing

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