High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study

M. Aires-De-Sousa, K. Boye, H. De Lencastre, A. Deplano, M. C. Enright, J. Etienne, A. Friedrich, D. Harmsen*, A. Holmes, X. W. Huijsdens, Angela Kearns, A. Mellmann, H. Meugnier, J. K. Rasheed, E. Spalburg, B. Strommenger, M. J. Struelens, F. C. Tenover, J. Thomas, U. VogelH. Westh, J. Xu, W. Witte

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

172 Citations (Scopus)

Abstract

Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.

Original languageEnglish
Pages (from-to)619-621
Number of pages3
JournalJournal of Clinical Microbiology
Volume44
Issue number2
DOIs
Publication statusPublished - Feb 2006

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