High-resolution analysis by whole-genome sequencing of an international lineage (Sequence Type 111) of pseudomonas aeruginosa associated with metallo-carbapenemases in the United Kingdom

Jane Turton*, Laura Wright, Anthony Underwood, Adam A. Witney, Yuen Ting Chan, Ali Al-Shahib, Catherine Arnold, Michel Doumith, Bharat Patel, Timothy D. Planche, Jonathan Green, Richard Holliman, Neil Woodford

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

Whole-genome sequencing (WGS) was carried out on 87 isolates of sequence type 111 (ST-111) of Pseudomonas aeruginosa collected between 2005 and 2014 from 65 patients and 12 environmental isolates from 24 hospital laboratories across the United Kingdom on an Illumina HiSeq instrument. Most isolates (73) carried VIM-2, but others carried IMP-1 or IMP-13 (5) or NDM-1 (1); one isolate had VIM-2 and IMP-18, and 7 carried no metallo-beta-lactamase (MBL) gene. Single nucleotide polymorphism analysis divided the isolates into distinct clusters; the NDM-1 isolate was an outlier, and the IMP isolates and 6/7 MBL-negative isolates clustered separately from the main set of 73 VIM-2 isolates. Within the VIM-2 set, there were at least 3 distinct clusters, including a tightly clustered set of isolates from 3 hospital laboratories consistent with an outbreak from a single introduction that was quickly brought under control and a much broader set dominated by isolates from a long-running outbreak in a London hospital likely seeded from an environmental source, requiring different control measures; isolates from 7 other hospital laboratories in London and southeast England were also included. Bayesian evolutionary analysis indicated that all the isolates shared a common ancestor dating back ≤50 years (1960s), with the main VIM-2 set separating approximately 20 to 30 years ago. Accessory gene profiling revealed blocks of genes associated with particular clusters, with some having high similarity (>95%) to bacteriophage genes. WGS of widely found international lineages such as ST-111 provides the necessary resolution to inform epidemiological investigations and intervention policies.

Original languageEnglish
Pages (from-to)2622-2631
Number of pages10
JournalJournal of Clinical Microbiology
Volume53
Issue number8
DOIs
Publication statusPublished - 1 Aug 2015

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Copyright © 2015, American Society for Microbiology. All Rights Reserved.

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