Pulsed-field gel electrophoresis (PFGE) was developed for separating and analyzing macrorestriction fragments of DNA in an alternating, homogenous electric field. Comparison of the electrophoretic banding profiles after digestion of intact bacterial DNA with restriction endonucleases is a useful epidemiological tool. Computer-assisted data analysis of PFGE subtypes has aided in many epidemiological studies and in tracing routes and sources of contamination in the food industry. For many foodborne disease infections, PFGE using a contour-clamped homogenous electric field (CHEF) is a still the gold standard for subtyping the implicated pathogens.
|Title of host publication||Encyclopedia of Food Microbiology|
|Subtitle of host publication||Second Edition|
|Number of pages||7|
|Publication status||Published - 2 Apr 2014|
- Alternating electrical field
- Foodborne pathogens
- Molecular epidemiology