Identification of group A streptococcal emm types commonly associated with invasive infections and antimicrobial resistance by the use of multiplex PCR and high-resolution melting analysis

P. Bidet, S. Liguori, C. Plainvert, S. Bonacorsi, C. Courroux, C. D'Humières, C. Poyart, Androulla Efstratiou, E. Bingen*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)

    Abstract

    M/emm typing, based either on serotyping of the M protein or on sequencing of the emm gene, is a major tool for epidemiological studies of group A streptococci (GAS). In order to simplify M/emm typing, we designed two multiplex polymerase chain reaction (PCR) formats capable of identifying the most frequent GAS M/emm types involved in invasive infections and antimicrobial resistance. A heptaplex PCR procedure was first developed in a conventional format coupled with gel electrophoresis to identify emm types 1, 3, 4, 6, 12, 28, and 89, based on the size of the amplification products. The other method, designed to identify the same seven emm types, together with emm11, was based on a real-time PCR format coupled with highresolution melting (HRM) analysis, allowing the rapid typing of large strain collections.

    Original languageEnglish
    Pages (from-to)2817-2826
    Number of pages10
    JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
    Volume31
    Issue number10
    DOIs
    Publication statusPublished - Oct 2012

    Bibliographical note

    Copyright:
    Copyright 2013 Elsevier B.V., All rights reserved.

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