In vitro transmission of chromosomal aberrations through mitosis in human lymphocytes

F. S. Pala, Jayne Moquet, Alan Edwards, D. C. Lloyd

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Stable and unstable chromosome aberrations in human lymphocytes exposed to 2 and 4Gy of X-rays in G0 were analyzed in M1 and M2 cells harvested at 72 h to investigate how the scoring protocol influences the yields of aberrations transmitted through one mitosis. Metaphase chromosomes 2, 3, and 5 were painted using fluorescence in situ hybridization (FISH) whole chromosome probes, together with a pan-centromeric probe and stained by the harlequin-FISH method, to allow the cell cycle status of each cell to be determined as it was scored. A strict scoring criterion was adopted so that each metaphase had to contain 46 centromeres and each dicentric/centric ring had to have an acentric present. In addition to scoring the painted material, unstable aberrations in the whole genome were also recorded. The yield of complete dicentrics decreased by more than a factor of 2 in going from M1 to M2. The decrease was greater at the lower dose. Two-way translocations appeared stable, but one-way translocations decreased. This suggests that if translocation yields are to be used for biological dosimetry purposes, then the two-way type should be used.

Original languageEnglish
Pages (from-to)139-146
Number of pages8
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume474
Issue number1-2
DOIs
Publication statusPublished - 1 Mar 2001

Keywords

  • Chromosomal aberrations
  • Mitotic division
  • Persistence

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