Investigation of hepatitis b virus transmission in a health care setting: Application of direct sequence analysis

M. A. Zuckerman, A. E. Hawkins, M. Briggs, J. Waite, P. Balfe, B. Thorn, R. J.C. Gilson, Richard Tedder

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

An epidemiologically linked cluster of hepatitis B virus (HBV) infections was investigated using HBV DNA amplification by a nested polymerase chain reaction with primers complementary to the region around the immunodominant a determinant of the surface gene, part of the X and core genes, and precore region and direct nucleotide sequence analysis. The cluster, in which 2 persons died of fulminant hepatitis, comprised 1 blood donor, 2 patients, and 2 health care workers. The Kimura two-parameter method was used to compare variance among the cluster with that in the control samples, which were collected from 7 patients infected with the same HBV subtype. Significantly less variation occurred within the cluster than in the control group (unpaired t test, P <.05). In an unrooted phylogenetic tree analysis, the 5 study samples formed a cluster distinct from the controls. This direct molecular approach of analyzing conserved regions of the HBV genome differentiated between viruses involved in HBV transmission events.

Original languageEnglish
Pages (from-to)1080-1083
Number of pages4
JournalJournal of Infectious Diseases
Volume172
Issue number4
DOIs
Publication statusPublished - Oct 1995

Bibliographical note

Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.

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