Investigation of the utility of the 1.1B4 cell as a model human beta cell line for study of persistent enteroviral infection

Jessica R. Chaffey, Jay Young, Kaiyven A. Leslie, Katie Partridge, Pouria Akhbari, Shalinee Dhayal, Jessica L. Hill, Kyle C.A. Wedgwood, Edward Burnett, Mark A. Russell*, Sarah J. Richardson, Noel G. Morgan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The generation of a human pancreatic beta cell line which reproduces the responses seen in primary beta cells, but is amenable to propagation in culture, has long been an important goal in diabetes research. This is particularly true for studies focussing on the role of enteroviral infection as a potential cause of beta-cell autoimmunity in type 1 diabetes. In the present work we made use of a clonal beta cell line (1.1B4) available from the European Collection of Authenticated Cell Cultures, which had been generated by the fusion of primary human beta-cells with a pancreatic ductal carcinoma cell, PANC-1. Our goal was to study the factors allowing the development and persistence of a chronic enteroviral infection in human beta-cells. Since PANC-1 cells have been reported to support persistent enteroviral infection, the hybrid 1.1B4 cells appeared to offer an ideal vehicle for our studies. In support of this, infection of the cells with a Coxsackie virus isolated originally from the pancreas of a child with type 1 diabetes, CVB4.E2, at a low multiplicity of infection, resulted in the development of a state of persistent infection. Investigation of the molecular mechanisms suggested that this response was facilitated by a number of unexpected outcomes including an apparent failure of the cells to up-regulate certain anti-viral response gene products in response to interferons. However, more detailed exploration revealed that this lack of response was restricted to molecular targets that were either activated by, or detected with, human-selective reagents. By contrast, and to our surprise, the cells were much more responsive to rodent-selective reagents. Using multiple approaches, we then established that populations of 1.1B4 cells are not homogeneous but that they contain a mixture of rodent and human cells. This was true both of our own cell stocks and those held by the European Collection of Authenticated Cell Cultures. In view of this unexpected finding, we developed a strategy to harvest, isolate and expand single cell clones from the heterogeneous population, which allowed us to establish colonies of 1.1B4 cells that were uniquely human (h1.1.B4). However, extensive analysis of the gene expression profiles, immunoreactive insulin content, regulated secretory pathways and the electrophysiological properties of these cells demonstrated that they did not retain the principal characteristics expected of human beta cells. Our data suggest that stocks of 1.1B4 cells should be evaluated carefully prior to their use as a model human beta-cell since they may not retain the phenotype expected of human beta-cells.

Original languageEnglish
Article number15624
JournalScientific Reports
Volume11
DOIs
Publication statusPublished - 2 Aug 2021

Bibliographical note

Funding Information: This work was supported by a JDRF Career Development Award (5-CDA-2014-221-A-N) to SJR, two JDRF research grants awarded to NGM & SJR as part of the network of Pancreatic Organ Donors – Virus (nPOD-V) consortium (JDRF 25-2012-516; JDRF-3-SRA-2017-492-A-N); an MRC Project Grant MR/P010695/1 awarded to SJR and NGM, a grant from the Norman Family Trust (to SJR & NGM) MRC Fellowship MR/P01478X/1 (to KCAW) a European Foundation for the Study of Diabetes (EFSD)/Lilly Fellowship to MAR and a DUK PhD Studentship (15/0005364) to NGM and SJR.

Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Publisher Copyright: © The Author(s) 2021

Citation: Chaffey, J.R., Young, J., Leslie, K.A. et al. Investigation of the utility of the 1.1B4 cell as a model human beta cell line for study of persistent enteroviral infection. Sci Rep 11, 15624 (2021).

DOI: https://doi.org/10.1038/s41598-021-94878-y

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