Is a semi-automated approach indicated in the application of the automated micronucleus assay for triage purposes?

H. Thierens*, A. Vral, C. Vandevoorde, V. Vandersickel, V. de Gelder, H. Romm, U. Oestreicher, Kai Rothkamm, Stephen Barnard, Elizabeth Ainsbury, S. Sommer, C. Beinke, A. Wojcik

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Within the EU MULTIBIODOSE project, the automated micronucleus (MN) assay was optimised for population triage in large-scale radiological emergencies. For MN scoring, two approaches were applied using the Metafer4 platform (MetaSystems, Germany): Fully automated scoring and semi-automated scoring with visual inspection of the gallery of MN-positive objects. Dose-response curves were established for acute and protracted whole-body and partial-body exposures. A database of background MN yields was set up, allowing determination of the dose detection threshold in both scoring modes. An analysis of the overdispersion of theMN frequency distribution σ2/μ obtained by semi-automated scoring showed that the value of this parameter represents a reliability check of the calculated equivalent total body dose in case the accident overexposure is a partial-body exposure. The elaborated methodology was validated in an accident training exercise. Overall, the semi-automated scoring procedure represents important added value to the automated MN assay.

Original languageEnglish
Article numberncu130
Pages (from-to)87-94
Number of pages8
JournalRadiation Protection Dosimetry
Volume159
Issue number1-4
DOIs
Publication statusPublished - Jun 2014

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