Isolation and characterisation of Vibrio cholerae from fish examined postmortem at ZSL London Zoo between 2014 and 2018

Edmund J. Flach*, Demi LeNette-Dawson, David Greig, Amina Ismail Ahmed, Claire Jenkins, Shinto K. John, Shaheed K. Macgregor, Nic Masters, Mark F. Stidworthy, Brian Zimmerman, Marie Anne Chattaway

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Background: When suspect Vibrio cholerae were cultured from fish at ZSL London Zoo, investigations were carried out to determine whether they were possible causes of cholera. Methods: Bacterial culture was carried out on fish examined postmortem and colonies were identified using standard techniques including the API 20NE biochemical test kits. Suspect isolates were submitted to the Public Health England laboratory for additional testing. Separately, a number of fish were submitted for routine histopathology. Results: On 13 occasions between 2014 and 2018, suspected V cholerae were cultured from individuals of eight different freshwater fish species. Archived cultures for eight of these (from six different fish species) were investigated and seven isolates (from five fish species) were confirmed as V cholerae, but all were non-O1, non-O139 strains. Whole-genome sequencing showed that the five fish species had unique V cholerae multilocus sequence types (three isolates from Aphanius danfordii were identical), all of which were genetically distant from human isolates. Conclusions: There was no evidence that these isolates could cause cholera. Histopathological changes consistent with vibriosis were seen in several fish, suggesting that V cholerae were causing the disease, but there were also concurrent infections or predisposing stress factors.

Original languageEnglish
Pages (from-to)e86
JournalVeterinary Record
Volume187
Issue number10
DOIs
Publication statusPublished - Nov 2020

Bibliographical note

Funding Information:
This work is based on a ZSL pathology internship research study carried out by DLND at ZSL London Zoo and Public Health England while undertaking her Professional Training Year of the BSc (Hons) Veterinary Biosciences course at the University of Surrey. The authors are indebted to the staff of the Aquarium at London Zoo and colleagues in the veterinary department (ZSL Wildlife Health Services) for help and advice regarding these cases, and to Emeritus Professor R Richards of the University of Stirling, Institute of Aquaculture, for the histopathological reports on some cases.

Publisher Copyright:
© 2020 British Veterinary Association

Keywords

  • Vibrio cholerae
  • aquatic organisms
  • fish
  • vibriosis
  • zoonoses

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