Lipid composition and transcriptional response of Mycobacterium tuberculosis grown under iron-limitation in continuous culture: Identification of a novel wax ester

Joanna Bacon*, Lynn G. Dover, Kim A. Hatch, Yi Zhang, Jessica M. Gomes, Sharon Kendall, Lorenz Wernisch, Neil G. Stoker, Philip D. Butcher, Gurdyal S. Besra, Phillip Marsh

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    59 Citations (Scopus)

    Abstract

    The low level of available iron in vivo is a major obstacle for microbial pathogens and is a stimulus for the expression of virulence genes. In this study, Mycobacterium tuberculosis H37Rv was grown aerobically in the presence of limited iron availability in chemostat culture to determine the physiological response of the organism to iron-limitation. A previously unidentified wax ester accumulated under iron-limited growth, and changes in the abundance of triacylglycerol and menaquinone were also observed between iron-replete and iron-limited chemostat cultures. DNA microarray analysis revealed differential expression of genes involved in glycerolipid metabolism and isoprenoid quinone biosynthesis, providing some insight into the underlying genetic changes that correlate with cell-wall lipid profiles of M. tuberculosis growing in an iron-limited environment.

    Original languageEnglish
    Pages (from-to)1435-1444
    Number of pages10
    JournalMicrobiology
    Volume153
    Issue number5
    DOIs
    Publication statusPublished - May 2007

    Bibliographical note

    Copyright:
    Copyright 2008 Elsevier B.V., All rights reserved.

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