Low specificity of the Murex fourth-generation HIV enzyme immunoassay in Tanzanian adolescents

Dean B. Everett, Helen A. Weiss, John Changalucha, Alessandra Anemona, Tobias Chirwa, David A. Ross, Deborah Watson-Jones, John Parry, Richard Hayes, David C. Mabey

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Objective: To determine the specificity of the Abbott Murex HIV antigen/antibody combination enzyme immunoassay (EIA) for the diagnosis of HIV infection in Tanzania. Methods: A cross-sectional survey of 7333 Tanzanian adolescents and young adults was carried out. Sera testing positive by the Murex assay were further evaluated using a battery of other EIA which detect either antibody to HIV-1 or p24 antigen, and by PCR using pol primers. Results: Of the 674 sera testing positive by the Murex assay, only 53 (7.9%) were confirmed. The specificity of the Murex assay was 91.5%. Conclusions: Serological tests for HIV may perform differently in different populations. New diagnostic tests should not be introduced into populations in which they have not been evaluated.

Original languageEnglish
Pages (from-to)1323-1326
Number of pages4
JournalTropical Medicine and International Health
Volume12
Issue number11
DOIs
Publication statusPublished - Nov 2007

Keywords

  • Africa
  • False positives
  • HIV tests
  • Specificity

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