Mass Spectral Analysis of Proteins of Nonculture and Cultured Viruses

Vlad Serafim, Nicola Hennessy, David J. Allen, Christopher Ring, Leonardo P. Munoz, Saheer Gharbia, Ajit J. Shah, Haroun N. Shah

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Nucleic-acid-based matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) applications for the detection and identification of viruses use specific or broad-range polymerase chain reaction (PCR) primers in combination with different post-PCR biochemistries. The most widely used biochemistry and variations thereof, a PCR/primer extension assay has been applied to the detection and genotyping of RNA and DNA viruses with a multiplexing level and throughput that support large-scale epidemiological research studies and might improve diagnosis of infections and co-infections in clinical applications. The study demonstrates the importance of genetic analysis and informative molecular markers for rapid and high-throughput epidemiological studies of pathogen transmission. In addition to the detection of viruses, PCR/primer extension assays on MALDI-TOF MS enable the characterization of viruses by genotyping. PCR/primer extension assays have successfully been applied to genotype Hepatitis B virus (HBV) and to detect up to 60 single-nucleotide drug resistance mutations in the HBV polymerase gene.

Original languageEnglish
Title of host publicationMALDI-TOF and Tandem MS for Clinical Microbiology
Publisherwiley
Pages197-209
Number of pages13
ISBN (Electronic)9781118960226
ISBN (Print)9781118960257
DOIs
Publication statusPublished - 15 Sep 2016

Keywords

  • Hepatitis B virus
  • MALDI-TOF MS
  • Microbial DNA analysis
  • Molecular detection
  • Polymerase chain reaction
  • Primer extension assays
  • Tandem MS
  • Viral genetic heterogeneity
  • Viral quantification
  • Viral transmission monitoring

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