Meningococcal transferrin-binding proteins A and B show cooperation in their binding kinetics for human transferrin

Russell H. Stokes, Jonathan S. Oakhill, Christopher L. Joannou, Andrew Gorringe, Robert W. Evans

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Neisseria meningitidis, a causative agent of bacterial meningitis and septicemia, obtains transferrin-bound iron by expressing two outer membrane-located transferrin-binding proteins, TbpA and TbpB. A novel system was developed to investigate the interaction between Tbps and human transferrin. Copurified TbpA-TbpB, recombined TbpA-TbpB, and individual TbpA and TbpB were reconstituted into liposomes and fused onto an HPA chip (BIAcore). All preparations formed stable monolayers, which, with the exception of TbpB, could be regenerated by removing bound transferrin. The ligand binding properties of these monolayers were characterized with surface plasmon resonance and shown to be specific for human transferrin. Kinetic data for diferric human transferrin binding showed that recombined TbpA-TbpB had Ka and Kd values similar to those of copurified TbpA-TbpB. Individual TbpA and TbpB also displayed Ka values similar to those of copurified TbpA-TbpB, but their Kd values were one order of magnitude higher. Chemical cross-linking studies revealed that TbpA and TbpB, in the absence of human transferrin, formed large complexes with TbpA as the predominant species. Upon human transferrin binding, a complex was formed with a molecular mass corresponding to that of a TbpB-human transferrin heterodimer as well as a higher-molecular-mass complex of this heterodimer cross-linked to TbpA. This indicates that TbpA and TbpB form a functional meningococcal receptor complex in which there is cooperativity in the human transferrin binding kinetics. However, iron loss from the diferric human transferrin-TbpA-TbpB complex was not greater than that from human transferrin alone, suggesting that additional meningococcal transport components are involved in the process of iron removal.

Original languageEnglish
Pages (from-to)944-952
Number of pages9
JournalInfection and Immunity
Volume73
Issue number2
DOIs
Publication statusPublished - Feb 2005

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