Methods for the Detection and Characterisation of Noroviruses Associated with Outbreaks of Gastroenteritis: Outbreaks Occurring in the North-West of England during Two Norovirus Seasons

Christopher Gallimore*, Jonathan Green, Alison F. Richards, Hillary Cotterill, Alan Curry, David Brown, Jim J. Gray

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

This article describes the methods used to investigate 407 outbreaks of acute non-bacterial gastroenteritis occurring in the North-West of England between January 2000 and July 2001 and suspected to be caused by noroviruses (NV) [Mayo (2002) Arch Virol 147:1655-1663]. These included 319 outbreaks in hospitals and nursing homes and 88 other settings. Eight hundred and seventy-one faecal samples from 407 outbreaks were tested using electron microscopy (EM), an enzyme-linked immunosorbent assay (ELISA) specific for Grimsby virus (GRV) capsid antigen and/or by reverse transcriptase-polymerase chain reaction (RT-PCR) for NV, allowing the utility of each assay for routine diagnosis to be assessed. Preliminary genomic characterisation of detected strains was performed using the heteroduplex mobility assay (HMA) and DNA sequencing. The results demonstrate the continuing predominance of GII-4 GRV strain of NV as a cause of outbreaks, particularly in hospital and nursing home settings. Overall, NV were detected in 223/407 (55%) of outbreaks tested. However, a wide range of apparently diverse strains was identified, including several not previously reported. Genomic characterisation revealed clusters of linked outbreaks not recognised previously.

Original languageEnglish
Pages (from-to)280-288
Number of pages9
JournalJournal of Medical Virology
Volume73
Issue number2
DOIs
Publication statusPublished - Jun 2004

Keywords

  • Electron microscopy
  • ELISA
  • Heteroduplex mobility assay
  • Human calicivirus
  • Molecular diversity
  • Norovirus
  • RT-PCR

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