Molecular characterization of Salmonella Typhimurium and Salmonella Enteritidis by plasmid analysis and pulsed-field gel electrophoresis

Zerrin Aktas*, Martin Day, Cigdem Bal Kayacan, Sukufe Diren, John Threlfall

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Forty-one paediatric isolates of Salmonella spp. from Cerrahpasa Faculty of Medicine, Istanbul, Turkey, between 2001 and 2004 were examined for susceptibility to various antibiotics and presence of antibiotic resistance genes. Pulsed-field gel electrophoresis and plasmid profiling were used to determine possible genetic relationships among Salmonella enterica subsp. enterica clinical isolates. Plasmids from resistant strains were not transferred by conjugation to recipient Escherichia coli cells. Pulsed-field gel electrophoresis and restriction enzyme digestion analysis of DNA revealed that multidrug-resistant isolates belonged to the same clonal group, characterized by ACSSuT resistotype. Isolates of R-type ACSSuT were positive for the intI gene and possessed a single plasmid of 60 MDa.

Original languageEnglish
Pages (from-to)541-545
Number of pages5
JournalInternational Journal of Antimicrobial Agents
Volume30
Issue number6
DOIs
Publication statusPublished - Dec 2007

Bibliographical note

Funding Information:
Funding: This study was supported by the Research Fund of the Istanbul University, project number UDP-516/15022007.

Keywords

  • ACSSuT
  • Plasmid profile analysis
  • Salmonella Enteritidis
  • Salmonella Typhimurium DT 104

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