Molecular diversity of Giardia duodenalis, cryptosporidium spp. And blastocystis sp. in asymptomatic school children in Leganés, Madrid (Spain)

Aly Salimo Muadica, Pamela Carolina Köster, Alejandro Dashti, Begoña Bailo, Marta Hernández-De-mingo, Lucia Reh, Sooria Balasegaram, Neville Verlander, Esther Ruiz Chércoles, David Carmena*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Enteric parasites including Giardia duodenalis, Cryptosporidium spp., and to a lesser extent, Blastocystis sp. and Enterocytozoon bieneusi, are major worldwide contributors to diarrhoeal disease. Assessing their molecular frequency and diversity is important to ascertain the sources of infection, transmission dynamics, and zoonotic potential. Little molecular information is available on the genotypes of these pathogens circulating in apparently healthy children. Here, we show that asymptomatic carriage of G. duodenalis (17.4%, 95% CI: 15.5‒19.4%), Blastocystis sp. (13.0%, 95% CI: 11.4‒14.8%), and Cryptosporidium spp. (0.9%, 95% CI: 0.5‒1.5%) is common in children (1‒16 years; n = 1512) from Madrid, Spain. Our genotyping data indicate that; (i) the observed frequency and diversity of parasite genetic variants are very similar to those previously identified in Spanish clinical samples, so that the genotype alone does not predict the clinical outcome of the infection, (ii) anthroponotic transmission accounts for a large proportion of the detected cases, highlighting that good personal hygiene practices are important to minimizing the risk of infection, (iii) Blastocystis ST4 may represent a subtype of the parasite with higher pathogenic potential, and (iv) Enterocytozoon bieneusi does not represent a public health concern in healthy children.

Original languageEnglish
Article number466
JournalMicroorganisms
Volume8
Issue number4
DOIs
Publication statusPublished - Apr 2020

Bibliographical note

Funding Information:
Funding: This research was funded by Health Institute Carlos III (ISCIII), Ministry of Science, Innovation and Universities, Spain, grant number PI16CIII/00024.

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Asymptomatic children
  • Blastocystis
  • Cryptosporidium
  • Enterocytozoon
  • Genotyping
  • Giardia
  • Molecular detection
  • Molecular epidemiology
  • PCR
  • Spain

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