Molecular typing of Neisseria gonorrhaeae causing repeated infections: Evolution of porin during passage within a community

Marcia M. Hobbs*, Timothy M. Alcorn, Rachael H. Davis, William Fischer, James C. Thomas, Iona Martin, Catherine Ison, P. F. Sparling, Myron S. Cohen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)

Abstract

Thirty-three Neisseria gonorrhoeae isolates from 15 persons infected multiple times with the same serovar were compared using pot gene sequencing, opa-typing, and arbitrarily primed-polymerase chain reaction. All three molecular techniques were more discriminatory than serotyping and identified differences between some isolates belonging to the same serovar. Although there were differences among Por sequences within some serovars, 10 of 15 subjects became reinfected with gonococci expressing identical Pot proteins. Sequence analysis of pot genes revealed evidence of horizontal genetic exchange and point mutations in potential surface-exposed regions during passage in the community.

Original languageEnglish
Pages (from-to)371-381
Number of pages11
JournalJournal of Infectious Diseases
Volume179
Issue number2
DOIs
Publication statusPublished - 1999

Bibliographical note

Funding Information:
Grant support: NIH (AI-31496; AI-38399 supports the Gonococcal Genome Sequencing Project) and Wellcome Trust.

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