Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae

on behalf of ESCMID Study Group for Mycoplasma Infections

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20 M. pneumoniae genomes per reaction.

Original languageEnglish
Pages (from-to)111-114
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Volume88
Issue number2
DOIs
Publication statusPublished - 1 Jun 2017

Bibliographical note

Publisher Copyright:
© 2017 Elsevier Inc.

Keywords

  • Detection
  • Multi-center evaluation
  • Mycoplasma pneumoniae
  • Real-time PCR

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