Multilocus genotyping of giardia duodenalis in mostly asymptomatic indigenous people from the tapirapé tribe, brazilian amazon

Pamela Carolina Köster, Antonio F. Malheiros, Jeffrey J. Shaw, Uthayasoori Balasegaram, Alexander Prendergast, Héloïse Lucaccioni, Luciana Melhorança Moreira, Larissa M.S. Lemos, Alejandro Dashti, Begoña Bailo, Arlei Marcili, Herbert Sousa Soares, Solange Maria Gennari, Rafael Calero-Bernal, David González-Barrio, David Carmena

Research output: Contribution to journalArticlepeer-review

Abstract

Little information is available on the occurrence and genetic variability of the diarrhoeacausing enteric protozoan parasite Giardia duodenalis in indigenous communities in Brazil. This cross-sectional epidemiological survey describes the frequency, genotypes, and risk associations for this pathogen in Tapirapé people (Brazilian Amazon) at four sampling campaigns during 2008- 2009. Microscopy was used as a screening test, and molecular (PCR and Sanger sequencing) assays targeting the small subunit ribosomal RNA, the glutamate dehydrogenase, the beta-giardin, and the triosephosphate isomerase genes as confirmatory/genotyping methods. Associations between G. duodenalis and sociodemographic and clinical variables were investigated using Chi-squared test and univariable/multivariable logistic regression models. Overall, 574 individuals belonging to six tribes participated in the study, with G. duodenalis prevalence rates varying from 13.5-21.7%. The infection was positively linked to younger age and tribe. Infected children <15 years old reported more frequent gastrointestinal symptoms compared to adults. Assemblage B accounted for three out of four G. duodenalis infections and showed a high genetic diversity. No association between assemblage and age or occurrence of diarrhoea was demonstrated. These data indicate that the most likely source of infection was anthropic and that different pathways (e.g., drinking water) may be involved in the transmission of the parasite.

Original languageEnglish
Article number206
Pages (from-to)1-32
Number of pages32
JournalPathogens
Volume10
Issue number2
DOIs
Publication statusPublished - Feb 2021

Keywords

  • Amazon
  • Asymptomatic
  • Brazil
  • Community
  • Genotyping
  • Giardia
  • Indigenous
  • Risk association
  • Tapirapé
  • Transmission

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