Multivalent liposome-based vaccines containing different serosubtypes of PorA protein induce cross-protective bactericidal immune responses against Neisseria meningitidis

Holly Humphries, Jeannette N. Williams, Ramona Blackstone, Keith A. Jolley, Ho M. Yuen, Myron Christodoulides, John E. Heckels*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Four serosubtypes (P1.7, 16, P1.7-2, 4, P1.19, 15 and P1.5-1, 10-4) of the PorA outer-membrane protein of Neisseria meningitidis were purified as recombinant proteins and incorporated into liposomes to investigate their immunogenicity. Each serosubtype induced high levels of bactericidal activity against the homologous strain. In addition, liposome preparations containing multiple serosubtypes induced high levels of bactericidal activity against each of the four strains. Significantly, antisera raised against monovalent and multivalent liposomes also showed cross-reactive bactericidal activity against heterologous strains. These data demonstrate that multivalent liposome vaccines, containing multiple PorA serosubtypes, have the potential to provide protection against a broad range of meningococcal strains.

Original languageEnglish
Pages (from-to)36-44
Number of pages9
JournalVaccine
Volume24
Issue number1
DOIs
Publication statusPublished - 9 Jan 2006
Externally publishedYes

Bibliographical note

Funding Information:
The work in this study was supported by The Meningitis Trust, The University of Southampton Strategic Development Fund and Hope (Wessex Medical Trust).

Keywords

  • Immunogenicity
  • Multivalent liposomes
  • Neisseria meningitides
  • Recombinant PorA

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