NB protein does not affect influenza B virus replication in vitro and is not required for replication in or transmission between ferrets

Ruth A. Elderfield, Marios Koutsakos, Rebecca Frise, Konrad Bradley, Jonathan Ashcroft, Shanhjahan Miah, Angie Lackenby, Wendy S. Barclay*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The influenza B virus encodes a unique protein, NB, a membrane protein whose function in the replication cycle is not, as yet, understood. We engineered a recombinant influenza B virus lacking NB expression, with no concomitant difference in expression or activity of viral neuraminidase (NA) protein, an important caveat since NA is encoded on the same segment and initiated from a start codon just 4 nt downstream of NB. Replication of the virus lacking NB was not different to wild-type virus with full-length NB in clonal immortalized or complex primary cell cultures. In the mouse model, virus lacking NB induced slightly lower IFN-α levels in infected lungs, but this did not affect virus titres or weight loss. In ferrets infected with a mixture of viruses that did or did not express NB, there was no fitness advantage for the virus that retained NB. Moreover, virus lacking NB protein was transmitted following respiratory droplet exposure of sentinel animals. These data suggest no role for NB in supporting replication or transmission in vivo in this animal model. The role of NB and the nature of selection to retain it in all natural influenza B viruses remain unclear.

Original languageEnglish
Pages (from-to)593-601
Number of pages9
JournalJournal of General Virology
Volume97
Issue number3
DOIs
Publication statusPublished - Mar 2016

Bibliographical note

Publisher Copyright:
© 2015 The Authors.

Fingerprint

Dive into the research topics of 'NB protein does not affect influenza B virus replication in vitro and is not required for replication in or transmission between ferrets'. Together they form a unique fingerprint.

Cite this